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Microsporum canis is distinguished from other dermatophytes within this genus by a hair perforation test. Microsporum canis shows a positive hair perforation test telling the observer that this type of dermatophyte grows into the hair shaft causing brittle breakable hairs. Another less sensitive test that can be used to diagnose Microsporum canis is the use of a Wood’s lamp, a fancy name for a certain wavelength of blacklight. Under the Wood’s lamp, Microsporum canis will fluoresce green, but only approximately 50% of the Microsporum canis strains will show fluorescence (1). After observation of either a positive hair perforation test or a positive Wood’s lamp test, the observer should then macroscopically and microscopically be able to distinguish this species from others due to the characteristcs mentioned in the description of the species above.
Microsporum canis is a dermatophyte found world wide causing tinea capitis and tinea corporis in humans. Microsporum canis has the ability to infect the keratinzed tissues such as the skin, hair and nails because of the virulent factor, keratinase(2). This species is zoophilic and the natural reservoir for Microsporum canis is dogs and cats, but can be equally virulent to humans, especially children. Humans generally contract ringworm through direct contact with an infected animal. To be sure this does not happen to you or your children, make sure your household pets, such as cats and dogs have been checked and treated for ringworm. If infected with Microsporum canis, griseofulvin is a drug that can be used, but less expensive and safer drugs have been developed such as oral terbinafine and intraconazole (1).
Microsporum canis, macroscopically, is a white to yellowish colony with a velvety/powdery texture approximately 3 to 9 cm in diameter. When the petri plate is flipped upside down, the colony ranges from being burnt orange to yellow depending on what strain of Microsporum canis may be growing on the plate(1).
Microsporum canis, microscopically, produces septate hyphae along with both macroconidia and microcondia. The Macroconidia are fusiform spindle-shaped approximately 35-125 X 7-20 micrometers in size. The macroconidia have thin septate walls within them with approximately 6-15 cells (1). The macroconidia’s outer walls tend to be very thick and rough. The microconidia also produced by this species are clavate and 4-7 X 2.5-3.5 micrometers in size (1).
As mentioned earlier, Microsporum canis can be found world wide infecting domestic cats and dogs, wild cats and dogs, humans and less commonly monkeys, horses, and rabbits. The only soil reported findings have been reported from sand beaches in Hawaii and soil in Romania (2).
Other taxa that may look similiar to Microsporum canis are other dermatophytes such as the genera Epidermophyton and Trichophyton. Although Microsporum species can be distinguished from these other two genera by having spindle-shaped macroconidia with rough enchinulate walls(3).
Microsporum canis is mostly a dermatophyte and can only be used by people for research as well as new and improved drug treatments. Although, maybe not Microsporum canis the species itself, but other dermatophytes are used by South American and African tribes as a sign of beauty. Some of the dermatophytes form geometrical shapes on the skin, which is known to be aesthetically pleasing in these tribes.
Microsporum canis is a pathogenic, asexual fungus in the phylum Ascomycota that infects the upper, dead layers of skin on domesticated cats, and occasionally dogs and humans.[1][2] The species has a worldwide distribution.[3]
Microsporum canis reproduces by means of two conidial forms, large, spindle-shaped, multicelled macroconidia and small, single-celled microconidia.[4][5] First records of M. canis date to 1902.[5] Evolutionary studies have established that M. canis, like the very closely related sibling species M. distortum and M. equinum, is a genetic clone derived from the sexually reproducing species, Arthroderma otae.[6] Members of Ascomycota often possess conspicuous asexual and sexual forms that can coexist in time and space. Microsporum canis exemplifies a common situation in ascomycetous fungi in which, over time, one mating type strain has undergone habitat divergence from the other and established a self-sustaining reproductive population that consists only of the asexual form.[3] It is hypothesized that asexual lineage of Microsporum canis evolved as a result of host-specific interactions, changes in ecological niche, as well as, geographic isolation of + and – mating types of Arthroderma otae, hence making it difficult to sustain sexual reproduction.[6] Early domestication of animals, such as cats and dogs, in Africa led to a later evolution of host-specific fungus, Microsporum canis, which is commonly associated with loose fur animals.[6] Nearly all reported isolates of Microsporum canis represent the "–" mating strain of A. otae.[6][7] Together with two closely related taxa, M. ferrugineum and M. audouinii, the clade is thought to have an African center of origin.[8]
Microsporum canis forms a white, coarsely fluffy spreading colony with a distinctive "hairy" or "feathery" texture.[4][5] On the underside of the growth medium, a characteristic deep yellow pigment develops due to the metabolites secreted by the fungus.[4][5] The intensity of this yellow pigmentation peak on the 6th day of colony growth and fades gradually making the identification of older colonies difficult.[4] Some strains of M. canis fail to produce yellow pigment altogether, exhibit abnormally slow colony growth and form undeveloped macroconidia.[4] Cultivation on polished rice tends to reestablish the typical growth morphology and is helpful for identification.[4]
Microsporum canis reproduces asexually by forming macroconidia that are asymmetrical, spherically shaped and have cell walls that are thick and coarsely roughened.[4][5] The interior portion of each macroconidium is typically divided into six or more compartments separated by broad cross-walls.[4][5] Microsporum canis also produces microconidia that resemble those of many other dermatophytes and thus are not a useful diagnostic feature.[3][4]
Microsporum canis produces infections of scalp and body sites, creating highly inflammatory lesions associated with hair loss.[3] Infection by this species can often be detected clinically using Wood's lamp, which causes infected tissues to fluoresce bright green[4][9] Fluorescence is attributed to metabolite pteridine, which is produced by the fungus in actively growing hairs.[3] Infected hairs remain fluorescent for prolonged periods of time (over the years), even after the death of the fungus.[3] Despite the frequent use of Wood's lamp in the clinical evaluation of ringworm infections, diagnosis of M. canis requires the performance of additional tests given the potential for false positives.[10] Culture of the fungus is most commonly used to evaluate morphological and physiological parameters of growth, and confirm the identity of the agent. Growth of the fungus on Sabouraud's agar (4% glucose), Mycosel or rice medium characteristically yields the bright yellow pigment. Microscopic examination of the growth can show the presence of the typical, warted and spindle-shaped macroconidia, confirming the identity of the isolate as M. canis.[11] The In vitro hair perforation test, commonly used to differentiate many dermatophytes, is not particularly useful for this species as it reveals the formation of "pegs" that penetrate into hair shafts - a characteristic shared widely among many zoophilic species.[5][12] Genetic analyses can be useful to establish the identity of atypical strains of M. canis; however the highly characteristic appearance of this species generally obviates the need for this more sophisticated method.[1][13]
Most M. canis infections are caused by the "-" mating strain of its sexual progenitor, Arthroderma otae.[3]
Microsporum canis has no specific growth factor or nutrition requirements, hence it grows well on most commercially available media.[5] In addition, M. canis exhibits rapid colony growth at 25 °C.[5] Two growth media that help distinguish M. canis from other Microsporum spp. (notably the morphologically similar species, M. audouinii) - specifically polished rice and potato dextrose agar.[4] On potato dextrose agar, M. canis produces a lemon-yellow pigment that is easily visualized, due to the presence of aerial hyphae, while on the polished rice, most isolates (even atypical strains) produce yellow pigment.[4][5]
It is considered a zoophilic dermatophyte, given that it typically colonizes the outer surface of animal's body.[4] Hence, animals, cats and dogs are believed to be the population hosts of this fungus, while humans are occasional hosts, in which the fungus can induce secondary infections.[4] Microsporum canis has been identified as a causal agent of a ringworm infection in pets, tinea capitis and tinea corporis in humans, children in particular.[2][3][5][14][15]
Microsporum canis is among the most common dermatophytes associated with tinea capitis and tinea corporis.[3][5] Unlike some dermatophyte species, M. canis typically does not cause large epidemics. Humans become infected as a result of direct or indirect contact with infected pets.[1][2][3] Microsporum canis generally invades hair and skin; however, some nail infections have been reported.[3] When hair shafts are infected, M. canis causes an ectothrix-type infection where the fungus envelopes the exterior of the hair shaft without the formation of internal spores.[4] This colonization of the hair shaft causes it to become unsheathed, resulting in characteristic round or oval non-inflammatory lesions the develop on the scalp.[4][9] Infection triggers an acute leukocytic reaction in subcutaneous tissues, which gradually becomes highly inflammatory and leads to hair loss, in the case of tinea capitis.[3]
Typically, infections caused by M. canis are associated with alopecia in the case of tinea capitis, while ringworm infections in pets produce characteristic inflammatory lesions, which may or may not result in hair loss.[3] This species has a propensity to cause subclinical infections in some animal species, particularly long-haired cats are frequent reservoirs of infection.[10] Isolation of the fungus from brushed pet hair can aid in detection of either an actively growing fungus or a passive carriage of fungal hyphae or arthroconidia.[10] In asymptomatic cases it is highly recommended to perform both Wood's lamp examination and microscopic analyses of suspected areas.[10][16] In the case of transient carriers, lack of clinical manifestations is accompanied with low number of M. canis colonies, number of which declines upon re-testing.[10]
Microsporum canis infections can be easily managed by topical antifungal agents; however severe cases may necessitate systemic therapy with griseofulvin, itraconazole or terbinafine.[1][9] Treatment of human cases also requires the identification and elimination of the infectious reservoir, which typically involves the investigation and treatment of colonized animals and the elimination of infected bedding and other environmental reservoirs.[10]
Despite its species name ("canis" implies dogs), the natural host of M. canis is the domestic cat. However this species can colonize dogs and horses as well. In all cases, it resides on the skin and fur.[10][13] Microsporum canis may also persist as dormant spores in the environment for prolonged periods.[10]
Microsporum canis species have a worldwide distribution.[1][3] Extremely high occurrence has been reported in Iran, while lower incidence is associated with England and Scandinavian countries, as well as South American countries.[3] Microsporum canis is uncommon in some parts of US and Europe, and is completely absent from equatorial Africa.[4]
Microsporum canis is a pathogenic, asexual fungus in the phylum Ascomycota that infects the upper, dead layers of skin on domesticated cats, and occasionally dogs and humans. The species has a worldwide distribution.
