Cycloneuralia

Description: English: A single adult female living specimen of Heligmosomoides bakeri was mounted on a microscope slide in Hanks's saline and sandwiched beneath a glass coverslip supported on petroleum jelly. The worm was imaged using a Zeiss Axiovert 135TV inverted microscope and photographed using a Scion CFW 1310 M digital camera. A solution of 25 μM papain was introduced below the coverslip and images were captured on a PC using Streampix III time-lapse software at a frame rate of approximately 1 image every 3 seconds for 30 minutes. The video file was edited and exported as an mpeg running at 10 times the original speed. The file is titled "H. bakeri female papain.mpg" and initially shows the worm freely moving in the papain solution. After the animal forms a helical coil, a lesion appears on the left of the worm. This is followed by rupture of the worm and loss of the viscera through the rupture leading to the death of the parasite. Date: 1 September 2008. Source: Behnke, J. M.; Buttle, D. J.; Stepek, G.; Lowe, A.; Duce, I. R. (2008). "Developing novel anthelmintics from plant cysteine proteinases". Parasites & Vectors 1: 29. doi:10.1186/1756-3305-1-29. Author: Behnke, J. M.; Buttle, D. J.; Stepek, G.; Lowe, A.; Duce, I. R. (2008). "Developing novel anthelmintics from plant cysteine proteinases". Parasites & Vectors 1: 29. doi:10.1186/1756-3305-1-29.

Description: English: Immune serum promotes adherence of BMMac to L4 larvae in vitro. Larvae recovered from the small intestine of challenge-infected mice were incubated with BMMac in the presence of or absence of immune serum for 24 h. Suspensions from co-cultures were transferred to glass slides, carefully covered with cover slips and time lapse-movies were immediately recorded with an Olympus AX70 microscope. Movies were processed using iMovie software. Representative examples are shown. Date: November 2013. Source: Movie S3 from Esser-von Bieren J, Mosconi I, Guiet R, Piersgilli A, Volpe B, Chen F, Gause W, Seitz A, Verbeek J, Harris N (2013). "Antibodies Trap Tissue Migrating Helminth Larvae and Prevent Tissue Damage by Driving IL-4R?-Independent Alternative Differentiation of Macrophages". PLOS Pathogens. DOI:10.1371/journal.ppat.1003771. PMID 24244174. PMC: 3828184. Author: Esser-von Bieren J, Mosconi I, Guiet R, Piersgilli A, Volpe B, Chen F, Gause W, Seitz A, Verbeek J, Harris N. Permission (Reusing this file): : This file is licensed under the Creative Commons Attribution 2.5 Generic license.:. You are free: to share – to copy, distribute and transmit the work to remix – to adapt the work Under the following conditions: attribution – You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/2.5 CC BY 2.5 Creative Commons Attribution 2.5 truetrue. This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.. Provenance: This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.: .

Description: English: The arginase inhibitor BEC prevents larval immobilization during challenge infection in vivo. C57BL/6 mice were treated with S-(2-boronoethyl)-L-cysteine (BEC) (0.2%, 100 µl by oral gavage) during challenge Hp infection. Mice were sacrificed on day 4 p.i. and small intestines were removed. Pieces of the upper duodenum were carefully covered with cover slips and time lapse-movies were immediately recorded with an Olympus AX70 microscope. Movies were processed using iMovie software. Representative examples are shown. Date: November 2013. Source: Movie S5 from Esser-von Bieren J, Mosconi I, Guiet R, Piersgilli A, Volpe B, Chen F, Gause W, Seitz A, Verbeek J, Harris N (2013). "Antibodies Trap Tissue Migrating Helminth Larvae and Prevent Tissue Damage by Driving IL-4R?-Independent Alternative Differentiation of Macrophages". PLOS Pathogens. DOI:10.1371/journal.ppat.1003771. PMID 24244174. PMC: 3828184. Author: Esser-von Bieren J, Mosconi I, Guiet R, Piersgilli A, Volpe B, Chen F, Gause W, Seitz A, Verbeek J, Harris N. Permission (Reusing this file): : This file is licensed under the Creative Commons Attribution 2.5 Generic license.:. You are free: to share – to copy, distribute and transmit the work to remix – to adapt the work Under the following conditions: attribution – You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/2.5 CC BY 2.5 Creative Commons Attribution 2.5 truetrue. This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.. Provenance: This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.: .

Description: English: Arg1 deficiency impairs immune serum induced trapping of larvae by BMMac in vitro. Larvae were incubated with BMMac from Arg1f/fTie2-Cre or Tie2-Cre mice in the presence of immune serum from challenge Hp infected C57BL/6 mice for 24 h. Suspensions from co-cultures were transferred to glass slides, carefully covered with cover slips and time lapse-movies were immediately recorded with an Olympus AX70 microscope. Movies were processed using iMovie software. Representative examples are shown. Date: November 2013. Source: Movie S4 from Esser-von Bieren J, Mosconi I, Guiet R, Piersgilli A, Volpe B, Chen F, Gause W, Seitz A, Verbeek J, Harris N (2013). "Antibodies Trap Tissue Migrating Helminth Larvae and Prevent Tissue Damage by Driving IL-4R?-Independent Alternative Differentiation of Macrophages". PLOS Pathogens. DOI:10.1371/journal.ppat.1003771. PMID 24244174. PMC: 3828184. Author: Esser-von Bieren J, Mosconi I, Guiet R, Piersgilli A, Volpe B, Chen F, Gause W, Seitz A, Verbeek J, Harris N. Permission (Reusing this file): : This file is licensed under the Creative Commons Attribution 2.5 Generic license.:. You are free: to share – to copy, distribute and transmit the work to remix – to adapt the work Under the following conditions: attribution – You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/2.5 CC BY 2.5 Creative Commons Attribution 2.5 truetrue. This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.. Provenance: This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.: .

Description: English: L-ornithine and polyamines reduce larval motility in vitro. Larvae were incubated in BMMac medium supplemented with L-ornithine or polyamines (100 µM) for 24 h, aliquots were transferred to glass slides and time lapse movies were recorded immediately with an Olympus AX70 microscope. Movies were processed using iMovie software. Representative examples are shown. Date: November 2013. Source: Movie S6 from Esser-von Bieren J, Mosconi I, Guiet R, Piersgilli A, Volpe B, Chen F, Gause W, Seitz A, Verbeek J, Harris N (2013). "Antibodies Trap Tissue Migrating Helminth Larvae and Prevent Tissue Damage by Driving IL-4R?-Independent Alternative Differentiation of Macrophages". PLOS Pathogens. DOI:10.1371/journal.ppat.1003771. PMID 24244174. PMC: 3828184. Author: Esser-von Bieren J, Mosconi I, Guiet R, Piersgilli A, Volpe B, Chen F, Gause W, Seitz A, Verbeek J, Harris N. Permission (Reusing this file): : This file is licensed under the Creative Commons Attribution 2.5 Generic license.:. You are free: to share – to copy, distribute and transmit the work to remix – to adapt the work Under the following conditions: attribution – You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/2.5 CC BY 2.5 Creative Commons Attribution 2.5 truetrue. This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.. Provenance: This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.: .