Raphidiophryidae

Raphidiophrys coerulea Scale bar indicates 25 µm. Sample from a pond situated in the vicinity of Lake Constance. The image was built up using several photomicrographic frames with manual stacking technique. Images were taken using Zeiss Universal with Olympus C7070 CCD camera.Image under Creative Commons License V 3.0 (CC BY-NC-SA). Place name: Pond situated in the vicinity of Lake Constance (Germany) Latitude: 47.734945 Longitude: 9.091097 Multiebenen-Abbildung, manuell gestapelt. Der Messbalken markiert eine Länge von 25 µm. Probe aus einem Teich in der Nähe des Bodensees. Mikrotechnik: Zeiss Universal, Kamera: Olympus C7070. Creative Commons License V 3.0 (CC BY-NC-SA). For permission to use of (high-resolution) images please contact postmaster@protisten.de.

Raphidiophrys elegans The picture shows the characteristic layer of mucilage armed with siliceous scales around the cell body and the narrow axopodia with numerous extrusomes. Sample from sphagnum pond situated in the northern alpine region of Austria near Salzburg. Images were taken using Zeiss Universal with Olympus C7070 CCD camera.Image under Creative Commons License V 3.0 (CC BY-NC-SA). Place name: Bogs near Salzburg (Austria) Latitude: 48.068516 Longitude: 12.954134 Das Bild zeigt die charakteristische Schleimhülle, verstärkt durch kieselige Schuppen, und die feinen Axopodien, die mit zahlreichen Extrusomen bewaffnet sind. Probe aus einem Moor in den nördlichen Kalkalpen von Österreich in der Nähe von Salzburg. Mikrotechnik: Zeiss Universal, Kamera: Olympus C7070. Creative Commons License V 3.0 (CC BY-NC-SA). For permission to use of (high-resolution) images please contact postmaster@protisten.de.

Raphidiophrys viridis Zoom-in showing one member of th colony with its silicious spiculae and the zoochlorellae. Scale bar indicates 100 µm. Sample from the pond Hegne Moor situated in the vicinity of Lake Constance. The image was built up using several photomicrographic frames with manual stacking technique. Images were taken using Zeiss Universal with Olympus C7070 CCD camera.Image under Creative Commons License V 3.0 (CC BY-NC-SA). Place name: Bog Hegne Moor near Lake Constance (Germany) Latitude: 47.718106 Longitude: 9.093974 Die Ausschnittsvergrößerung zeigt die SIlikatschuppen und die Zoochlorellen. Multiebenen-Abbildung, manuell gestapelt. Der Messbalken markiert eine Länge von 100 µm. Probe aus dem Simmelried nahe Konstanz. Mikrotechnik: Zeiss Universal, Kamera: Olympus C7070. Creative Commons License V 3.0 (CC BY-NC-SA). For permission to use of (high-resolution) images please contact postmaster@protisten.de.

Raphidiophrys viridis Colony. Scale bar indicates 100 µm. Sample from the pond Hegne Moor situated in the vicinity of Lake Constance. The image was built up using several photomicrographic frames with manual stacking technique. Images were taken using Zeiss Universal with Olympus C7070 CCD camera.Image under Creative Commons License V 3.0 (CC BY-NC-SA). Place name: Bog Hegne Moor near Lake Constance (Germany) Latitude: 47.718106 Longitude: 9.093974 Zellkolonie. Multiebenen-Abbildung, manuell gestapelt. Der Messbalken markiert eine Länge von 100 µm. Probe aus dem Simmelried nahe Konstanz. Mikrotechnik: Zeiss Universal, Kamera: Olympus C7070. Creative Commons License V 3.0 (CC BY-NC-SA). For permission to use of (high-resolution) images please contact postmaster@protisten.de.

Raphidiophrys capitata Scale bar indicates 50 µm.Sample from a tropical freshwater aquarium. Images were taken using Zeiss Axioplan with Olympus OM-D M5 MKII.Image under Creative Commons License V 3.0 (CC BY-NC-SA). Place name: Tropical freshwater aquarium Latitude: 54.3018013 Longitude: 10.07120132 Multiebenen-Abbildung, manuell gestapelt. Der Messbalken markiert eine Länge von 50 µm. Probe aus einem Süßwasseraquarium für tropische Fische. Mikrotechnik: Zeiss Axioplan, Kamera: Olympus OM-D M5 MKII. Creative Commons License V 3.0 (CC BY-NC-SA). For permission to use of (high-resolution) images please contact postmaster@protisten.de.

Raphidiophrys capitata Close-up with silicious scales. Sample from an on-site preflooder on the island of Hiddensee south of the village Kloster. Sampling date 9/2022. Images were taken using Zeiss Universal with Olympus OM-D M5 MKII.Image under Creative Commons License V 3.0 (CC BY-NC-ND). Place name: Preflooder south of the village Kloster, Hiddensee (German Baltic Sea) Latitude: 54.57611642 Longitude: 13.11450005 Ausschnitt mit Silikatschuppen. Probe aus einem Bodden-Vorfluter auf Hiddensee südlich von Kloster. Datum der Aufsammlung: 9/2022. Mikrotechnik: Zeiss Universal, Kamera: Olympus OM-D M5 MKII. Creative Commons License V 3.0 (CC BY-NC-ND). For permission to use of (high-resolution) images please contact postmaster@protisten.de.

Raphidiophrys capitata Scale bar indicates 25 µm. Collected from a pond near the Bodden bay Schwarzer Peter in the southern part of the isle Hiddensee (German Baltic Sea). Sampling date 10/2018. The image was built up using several photomicrographic frames with manual stacking technique. Images were taken using Zeiss Standard with Olympus OM-D M5 MKII. Image under Creative Commons License V 3.0 (CC BY-NC-SA). Place name: Pond near the Bodden bay Schwarzer Peter, Hiddensee (German Baltic Sea) Latitude: 54.514946 Longitude: 13.082471 Multiebenen-Abbildung, manuell gestapelt. Der Messbalken markiert eine Länge von 25 µm. Probe aus einem Teich nahe der Boddenbucht Schwarzer Peter auf Hiddensee. Datum der Aufsammlung: 10/2018. Mikrotechnik: Zeiss Standard, Kamera: Olympus OM-D M5 MKII. Creative Commons License V 3.0 (CC BY-NC-SA). For permission to use of (high-resolution) images please contact postmaster@protisten.de.

Raphidiophrys capitata Scale bar indicates 25 µm. Sample from an on-site preflooder on the island of Hiddensee south of the village Kloster. Sampling date 9/2022. Images were taken using Zeiss Universal with Olympus OM-D M5 MKII.Image under Creative Commons License V 3.0 (CC BY-NC-ND). Place name: Preflooder south of the village Kloster, Hiddensee (German Baltic Sea) Latitude: 54.57611642 Longitude: 13.11450005 Der Messbalken markiert eine Länge von 25 µm. Probe aus einem Bodden-Vorfluter auf Hiddensee südlich von Kloster. Datum der Aufsammlung: 9/2022. Mikrotechnik: Zeiss Universal, Kamera: Olympus OM-D M5 MKII. Creative Commons License V 3.0 (CC BY-NC-ND). For permission to use of (high-resolution) images please contact postmaster@protisten.de.

Raphidiophrys capitata Scale bar indicates 50 µm.Sample from a tropical freshwater aquarium. Images were taken using Zeiss Axioplan with Olympus OM-D M5 MKII.Image under Creative Commons License V 3.0 (CC BY-NC-SA). Place name: Tropical freshwater aquarium Latitude: 54.3018013 Longitude: 10.07120132 Multiebenen-Abbildung, manuell gestapelt. Der Messbalken markiert eine Länge von 50 µm. Probe aus einem Süßwasseraquarium für tropische Fische. Mikrotechnik: Zeiss Axioplan, Kamera: Olympus OM-D M5 MKII. Creative Commons License V 3.0 (CC BY-NC-SA). For permission to use of (high-resolution) images please contact postmaster@protisten.de.

Madrid, Madrid, Spain

Galende, Castile and Len, Spain

Galende, Castile and Len, Spain

Muelas del Pan, Castille and Leon, Spain

Lumbreras, La Rioja, Spain

Galende, Castile and Len, Spain

Galende, Castile and Len, Spain

Galende, Castile and Len, Spain

Ribadelago de Franco, Castille and Leon, Spain

Ribadelago, Castille and Leon, Spain

Ribadelago de Franco, Castilla y Len, Espaa

Ribadelago de Franco, Castille and Leon, Spain

Raphidiophrys (raff-fid-ee-off-riss) ambigua is a solitary species in this genus of centrohelid heliozoa. It forms three kinds of spindle shaped spicules that accumulate around the pseudopodia. The first form are spindle shaped and pointed at both ends, the second form are medium sized, broadly spindle shaped and rounded at the ends, and the final form are very small (5 - 6 mm long) and elliptically shaped. The spicules are embedded in a gelatinous envelope of the cell. Cytoplasm without symbiotic algae but often coloured greenish, brownish or yellowish by food vacuoles. Three closely arranged specimen of Raphidiophrys ambigua, possibly shortly after cell division. Note the yellow brownish food vacuoles that are characteristic of this species. From a pond near Konstanz, Germany.Differential interference contrast.

Single whole plate scale viewed by transmission electron microscopy. The siliceous scales are formed within the cell and then form a loose layer or periplast around the outside of the cell.

Composite image showing single scales (upper left). Upper left corner treated with hydrofluoric acid to show siliceous nature, vertical scale from trophic cell (Nomarski image upper right corner), larger scale from the cyst (middle right image). The lower image is a transmission electron micrograph of a thin section showing the scales adhering to the outer surface of the cell.