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FIGS. 1-7. Ostreopsis labens sp. nov. FIGS. 1-3. Light microscope views. Scale bar = 25 μm. Cells contain chloroplasts and a spherical posterior nucleus (n). FIG.1. Cell is in epithecal view. FIG. 2. Location of nucleus stained with DAPI. FIG.3. Hypothecal plates partially separated with numerous pores. Cell with an engulfed prey organism (arrowhead); red color not detected on a black and white print. FIGS. 4-7. Cells viewed with SEM. FIG. 4. Cell is broadly ovoid in epithecal view. Note the curved, long apical pore (Po) located off-center (arrow). Scale bar =10 µm. FIG. 5. Cell is in hypothecal view. Cell is smooth with scattered pores (arrows). Scale bar =10µm. FIG. 6. Cell is slightly convex in lateral view. Note lipped, equatorial cingulum (arrowheads). Scale bar =10 µm. FIG. 7. Antapical plate 1"is with a slightly curved list (arrowhead). The sulcus narrow, recessed and hidden adjacent to plate 2". The ventral opening (arrow) is situated on the ventral surface adjacent to a ridged plate (Rp) (asterisk). Scale bar = 5 μm. EMu: : HOLOTYPE SEM NEGATIVE # 170058; SEM STUB # 170; FIELD # 745-94; ACCESSION # 410840; CATALOG # 984; FIGURE # 4
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FIGS. 8-13. Ostreopsis labens sp. nov. FIG. 8. Ventral view is showing bi-convexity of the cell. Scale bar = 25 µm. FIG. 9. The 2" is very small (arrowhead). The ventral opening with a protuberant ridge and a curved plate (Rp) are situated in the cingulum adjacent to plate 1" and plate 1". Cingulum is smooth. Scale bar = 5 µm. FIG. 10. The ventral opening (Vo) is situated on the ventral plate (Vp). Scale bar = 5 µm. FIG. 11. Thecal surface is smooth; evenly spaced around trichocyst pores with smooth edges. Scale bar = 5 µm. FIG. 12. The apical pore (Po) is long, curved, and narrow associated with plate 2'. Row of marginal pores similar in size to thecal pores. Scale bar = 5 µm. FIG. 13. Right ventral view is unusual, recessed in sulcus. Flagellar pore opening is narrow (arrow). EMu: : HOLOTYPE SEM NEGATIVE # 170058; SEM STUB # 170; FIELD # 745-94; ACCESSION # 410840; CATALOG # 984; FIGURE # 4
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FIGS. 14, 15. Ostreopsis labens sp. nov. FIG. 14. Surface of cingulum is smooth, deep and narrow with equally spaced round pores (arrowheads). Scale bar = 2 μm. FIG. 15. The inner cell surface is smooth; thecal plate relatively thick with round trichocyst pores (arrows). Scale bar = 2 µm. EMu: : HOLOTYPE SEM NEGATIVE # 170058; SEM STUB # 170; FIELD # 745-94; ACCESSION # 410840; CATALOG # 984; FIGURE # 4
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Plate 32. Ostreopsis lenticularis. Figs. 1-5. SEM. Fig. 1. Epithecal view: cell lenticulate to broadly oval. Curved off-center apical pore plate with a slit-like apical pore (arrow). Plate 1' irregularly pentagonal. Fig. 2. Hypothecal view: plate 1p central and pentagonal. Fig. 3. Smooth thecal surface. Round pores with smooth raised edges. Fig. 4. Hypothecal ventral view: cell anterio-posteriorly compressed. Shallow cingulum with smooth edge. Small sulcus hidden (arrow). Fig. 5. Location of ventral opening (arrow), ventral plate (asterisk), and rigid plate (arrowheads) within cingulum. Fig. 6. Line drawing: thecal plate arrangement. Figs. 7,8. LM. Fig. 7. Cytoplasma granulated; posterior nucleus (n). Fig. 8. Distinct cingular list.
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Figs 1-5. Cells of Ostreopsis marinus sp. nov. Figs 1-4. Scanning electron microscopy. Fig. 1. Morphology of epithecal plates and position of apical pore plate are shown (Po). Fig. 2. Hypothecal plates. Fig. 3. Antapical plate 1" is larger; plate 2" is tiny. Thecal surface is smooth with small, evenly distributed pores (arrows). Fig. 4. The ventral opening (Vo) is situated in the cingulum adjacent to a ridged plate (Rp). Fig. 5. Epifluorescence light microscopy of hypothecal plates; Ip plate is in the center.
EMu: HOLOTYPE SEM NEGATIVE # 212055; SEM STUB # 212; FIELD # 96/10; ACCESSION # 2002799; CATALOG #1537; FIGURE # 1.
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Plate 33. Ostreopsis mascarenensis. Figs. 1-5. SEM. Fig. 1. Epitheca: inner thecal surface. Cell very large, broadly ovate, large plates. Plate 1' elongate and hexagonal. Apical pore plate (Po) nearly straight. Fig. 2. Hypotheca: plate 1p long and wide. Fig. 3. Smooth cell surface with round pores; pores with two small openings (arrows). Fig. 4. Po with long narrow apical pore; small pores line the opening (arrowheads). Figs. 5-6. Ventral view of epitheca. Fig. 5. Cell compressed anterio-posteriorly; cingulum narrow with smooth edge. Small sulcus hidden (arrow). Fig. 6. Location of ventral opening (large arrow), ventral plate (asterisk), and rigid plate (arrowheads) within cingulum. Pores with ejected trichocysts (small arrows). Fig. 7. LM. Epitheca: Po (arrow) and cingulum in focus. Fig. 8. Line drawing: hypotheca plate arrangement.
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Plate 34. Ostreopsis ovata. Figs. 1-5. SEM. Fig. 1. Epithecal view: cell slender and tear-shaped. Apical pore plate (Po) off-center (arrow). Plate 1' large and hexagonal. Cingulum wide with narrow lists. Fig. 2. Hypothecal view: plates delicate. Plate 1p long and narrow. Fig. 3. Po: short and straight, adjacent to plate 2'. Fig. 4. Thecal surface smooth with scattered small pores. Suture line uneven and bumpy (arrows). Fig. 5. Hypothecal view: ventral opening (arrow), ventral plate (asterisk), and rigid plate (arrowhead) on cingulum. Fig. 6. LM. Large posterior nucleus. Fig. 7. Line drawing: thecal plate arrangement.
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Plate 35. Ostreopsis siamensis. Figs. 1-6. SEM. Fig. 1. Epithecal view: cell broad and tear-shaped. Thecal surface smooth with scattered pores. Apical pore plate (Po) off-center (arrow). Narrow cingulum with smooth edge. Plate 1' narrow and pentagonal. Fig. 2. Hypothecal view: plate 1p long and pentagonal. Fig. 3. Po: long, curved and narrow. Fig. 4. Large and small pores on thecal surface. Fig. 5. Ventral view: location of ventral opening (arrow), ventral plate (asterisk), and rigid plate (arrowhead) on cingulum. Fig. 6. Hypothecal view: Vo (arrow) and Rp (arrowhead). Fig. 7. LM. Hypotheca. Fig. 8. Line drawing: thecal plate arrangement.
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Plate 36. Pfiesteria piscicida: Figs. 1-4. SEM. Figs. 1-2. Biflagellated stage resembles a gymnodinioid cell. Cells small, oblong and thecated. Fig. 1. Plate sutures apparent (arrows). Both flagella present (arrowheads). Fig. 2. Peduncle deployed (arrow). Fig. 3. Biflagellated stage with 2 size groups: large vegetative (V) cell; small gamete (G) cell. Fig. 4. Flagellated stage. Planozygote: larger triflagellated Plate 36. Pfiesteria piscicida: Figs. 1-4. SEM. Figs. 1-2. Biflagellated stage resembles a gymnodinioid cell. Cells small, oblong and thecated. Fig. 1. Plate sutures apparent (arrows). Both flagella present (arrowheads). Fig. 2. Peduncle deployed (arrow). Fig. 3. Biflagellated stage with 2 size groups: large vegetative (V) cell; small gamete (G) cell. Fig. 4. Flagellated stage. Planozygote: larger triflagellated stage; similar to vegetative cell with 2 longitudinal flagella (arrows) adjacent to peduncle (P). Fig. 5. LM. Triple layer cyst (arrows): benthic stage. Nucleus (N) stained with DAPI (courtesy of P. Tester). Figs. 6-7. SEM: biflagellate stage. Fig. 6. Epithecal plate morphology: thecal nodules apparent. Small 1a plate triangular (arrowhead). Plate 1' rhomboid (after Steidinger et al. 1996). Fig. 7. Apical view of APC: Po (arrowhead), cp, X plate. Figs. 8-9. Line drawings: plate tabulation. Fig. 8. Apical view: epitheca. Fig. 9. Ventral view: thecal nodules depicted.
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Figs 1-6. Plagiodinium belizeanum sp. nov. FIGS. 1-2. Light microscope view. Cells contain chloroplasts, spherical starch bodies (arrowheads) and a spherical posterior nucleus (n). FIG. 1. Cell is in left lateral view. FIG. 2. Cell is in right lateral view. FIGS.3-4. Cells viewed with the scanning electron microscope. FIG. 3. Cell is in left lateral view. FIG. 4. Cell is in right lateral view. Note an inclined, small, cap-shaped epitheca, deep cingulum, and an oblong hypotheca. The thecal surface is smooth. The large hypothecal plates 1'", 2'", 3'" (Fig. 3), and 4'" (Fig. 4) and antapical plate 1" are indicated. FIG. 5. The antapical 1" plate has a convex posterior shape. Small scattered thecal pores (arrowheads) are present. FIG. 6. Apical view of the epitheca. Epitheca narrowly oval pointed ventrally and rounded dorsally. The cingulum is deep, and the anterior and posterior margins are thickened. The cingulum has five plates (C1-C5) and is more depressed at its dorsal side. The hypothecal plates 1'", 2'", and 3'" are shown.
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Figs 7-12. Plagiodinium belizeanum sp. nov. FIG. 7. Cell is in ventral view. Two narrow plates 1'" and 5'" are present slightly displaced. The left ventral plate (‘’’) has an anterior indentation limited to the left by a longitudinal ridge extending posteriorly, decreasing in height, and ending at the middle of the plate. The sulcus is very small. The antapical (1’’’’) plate is convexed posteriorly with a pointed tip in the center of the ventral margin. FIG. 8. Cell is in dorsal view. Hypotheca is oblong and bilaterally flattened. Plates 3'" and 4'" and antapical 1" are separated by wide intercalary bands (arrowheads). FIG. 9. Dorsal view of epitheca. The shape of the epitheca is oblong, undifferentiated (arrow), and reduced in size relative to the hypotheca. Cingulum is broad. FIG. 10. Thecal surface is smooth with scattered pores. Intercalary band delineates plates 4'" and 5'" in the form of a distinct suture. FIG. 11. Inside view of the theca. The inner cell surface is smooth; thecal plates are relatively thick with a distinct intercalary band. Dorsal plate 3'" is convex and attached to plate 4'" (arrowheads). FIG. 12. Inside view of the intercalary band. At this magnification the cell's intercalary band is broadly striated inside (arrows) and outside (arrowheads).
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Figs 13-20. Line drawings of thecal plates of Plagiodinium belizeanum sp. nov. FIG. 13. Left lateral view; FIG. 14. Ventral view; FIG. 15. Antapical view; FIGS. 16. a, b) Plate composition of the epitheca and cingulum. Architecture of epithecal and cingular plates in a left view and right view; FIG. 17. Cross-section of the epitheca (a), cingulum (c), and hypotheca (h); A schematic representation of a section of a cell along the midplane parallel to the ventral surface. FIG. 18. Shape position and designation of plates. Relationship on epitheca plates is: (1’-5' and Po) cingulum (C1-C5), and sulcus (S.a.). A minute thecal element (PO), perhaps the 6th epithecal plate situated by the ventral internal border of the 4' plate, probably represents the rudiment of the PO plate. FIG. 19. Hypothecal plates (1'"-5'") and antapical plate 1"). FIG. 20. Designation, shape, and location of five sulcal plates: S.a., S.d., S.p., S.s., and S.m., surrounded by cingular plates Cl and C5 and epithecal plates 1' and 5'.
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Figs 21-22. Plagiodinium belizeanum sp. nov. with flagella. FIG. 21. The longitudinal flagellum (arrowhead) and the transverse flagellum (arrow) originate within the sulcal region. The longitudinal flagellum is approximately 30 µm long and the transverse flagellum is undulate and displaced. FIG. 22. Both flagella appear to originate at close proximity to one another (arrowhead).
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Figs. 14-19. Prorocentrum arenarium sp. nov. FIG.14. Cells in right valve view are round to oval. Cell distorted due to preparation. The periflagellar area is a broad, V-shaped depression. The longitudinal short flagellum is visible (arrowhead). Marginal poroids (arrows). FIG.15. The valve surface in left valve view is smooth and scattered with valve and marginal poroids (arrows). FIG. 6. The oblique ventral view is ellipsoid. The intercalary band is smooth. The marginal poroids are evenly spaced (arrows). FIG.17. The periflagellar area is triangular and unornamented. It has a large flagellar pore (F) and one smaller auxiliary pore (A). The apical platelets appear vertical when viewed from the anterior end of the cell. FIG. 18. Both flagella emerge from the flagellar pore (F): longitudinal flagellum (arrowheads), transverse flagellum (arrows). FIG. 9. At higher magnification, valve and marginal poroids are kidney-shaped to oblong (arrowheads) and are similar in size.FIGS. 20-21. Prorocentrum arenarium sp. nov. with peduncle-like structures. FIG. 20. The periflagellar area exhibits a short flagellum 11µm long (arrows), and a short, curved tubular structure 2-3 µm long (arrowheads), both emerging from the flagellar pore (F). The width of the curved tubular structure and flagellum are the same. FIG. 21. Both tubular structures emerge from the flagellar pore (F); one is straight, 2 µm long (arrow), and the other curved, 3µm long (arrowheads). The auxiliary pore (A) is to the left of the flagellar pore.
EMu: HOLOTYPE SEM NEGATIVE #133024; SEM stub # 133; Field # 556-92; Accession # 407166; Catalog # 97; Figure # 14.
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Plate 37. Prorocentrum arenarium. Figs. 1-5. SEM. Fig. 1. Right valve: cells round to ovoid. Periflagellar area is a broad, V-shaped depression. Short longitudinal flagellum visible (arrowhead). Marginal poroids present (arrows). Fig. 2. Left valve: surface smooth, with scattered valve and marginal poroids (arrows). Fig. 3. Lateral view: intercalary band smooth; marginal poroids evenly spaced (arrowheads). Fig.4. Marginal poroids oblong to kidney-shaped. Fig. 5. Periflagellar area: triangular and unornamented with large flagellar pore (f) and smaller auxiliary pore (a). Fig. 6. LM. Right valve: posterior nucleus (n) and prominent central pyrenoid (arrow).
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Plate 38. Prorocentrum balticum. Figs. 1-3. SEM. Fig. 1. Valve view: cell round to spherical, covered with many tiny spines. Apical spine apparent. Intercalary band broad, transversely striated (arrows). Fig. 2. Surface with scattered rimmed pores (arrows). Fig. 3. Periflagellar region: two different sized pores and two small apical projections (arrows). Fig. 4. Line drawing.
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Figs. 1-10. Prorocentrum belizeanum sp. nov. Fig.1. Valve surface is areolated. Fig.2. Cells are round to oval in valve view. Fig.3. In side view, cells are ellipsoid, the apical area exhibits a rounded lip, and both left and right valves are excavated. Fig.4. Periflagellar area is a wide, V-shaped depression located in the right valve. It has a flagellar and auxiliary pore, equal in size. Fig.5. Auxiliary pore (A) is surrounded by a curved apical collar (arrowheads) and is adjacent to the flagellar pore (F). The left valve margin exhibits a wide, rounded ridge (arrow). The two flagella are not shown. Fig.6. Intercalary band is horizontally striated. Fig.7. Areolae are round to ovoid with a smooth margin. Some areolae have trichocyst pores (arrowheads). Fig.8 . The center of the inside valve surface is smooth. The arrangement of round trichocyst pores is shown. Fig. 9. Inside valve surface shown at higher magnification. Trichocyst pores (arrowheads) are arranged in an array along the intercalary band. Fig.10. Inside of the intercalary band is constructed of evenly spaced rectangular sections separated by shallow grooves. Scale bars =10 µm.
EMu: Holotype SEM negative # 104052; SEM stub #104; Field # n.a; Accession # 407164; Catalog # 34; Figure # 1.
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Plate 39. Prorocentrum belizeanum. Figs. 1-6. SEM. Fig. 1. Right valve: cell round to oval; surface heavily areolated. Fig. 2. Left valve: anterior margin with flared curved apical collar. Marginal areolae visible. Fig. 3. Lateral view: valve center concave; intercalary band smooth and wide. Fig. 4. Apical view: apical area with rounded lip; both valves excavated. Fig. 5. Areolae round to ovoid with smooth margins; some with pores. Fig. 6. Periflagellar area: auxiliary pore (a) surrounded by curved periflagellar collar (arrows); adjacent to flagellar pore (f). Left valve with flared apical collar (arrowheads). Fig. 7. Left valve: central pyrenoid (arrow) and posterior nucleus (n). Fig. 8. LM: right valve; flagella present. Fig. 9. Line drawing: areolae arrangement (after Faust 1993a).
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Figs. 17-27. Prorocentrum caribbaeum sp. nov. FIG.17. Cell shape is oval with a rounded anterior and a pointed posterior end. FIG.18. Valve surface is smooth with minute depressions. Radially arranged trichocyst pores are present on each valve. The two flagella are not shown. FIG.19. Cells are ovate to convex in side view. The periflagellar area is located in the right valve and is highly ornate. FIG.20. In valve view, the periflagellar area has a rectangular orientation and is composed of a curved apical collar (on the left) and a smaller protuberant apical plate (on the right). FIG.21. Curved apical collar (arrow) is the largest platelet situated adjacent to the auxiliary pore (A). The apical plate (arrowhead) is located next to the flagellar pore (F) and is separated by a rectangular platelet from the auxiliary pore. FIG.22. The trichocyst pores (arrow) are round with smooth edges and are similar in size. They are situated in furrowed depressions. Small, round pores (arrowhead) are also present, unevenly distributed on the valve surface. FIG.23. The posterior end is pointed and laced with trichocyst pores and small, round pores unique to this species. FIG.24.Ejected trichocysts are on valve surface. FIG.25. The intercalary band is transversally striated and sinous. FIG.6.Inner valve surface is smooth. The location of round trichocyst pores is illustrated, and a distinct striated intercalary band is present (arrowheads). FIG. 7. The inner face of the intercalary band is highly ornate and lacelike. Scale bars = 8 µm.
EMu: Holotype SEM negative # 103001; SEM stub #103; Field # 358-90 Accession # 407164; Catalog # 49; Figure # 17.
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Plate 40. Prorocentrum concavum. Figs. 1-4. SEM. Fig. 1. Right valve. Cell ovate and heavily areolate. Valve center devoid of areolae. Left valve with anterior apical ridge (arrowhead). Fig. 2. Lateral view. Valve center concave and flattened. Intercalary band granulated and horizontally striated. Fig. 3. Valve areolae round to oval with smooth edges; some with small central pores. Fig. 4. Periflagellar area a V-shaped depression. Two pores: small auxiliary pore (a); large flagellar pore (f). Figs. 5-6. LM (M.A. Faust). Fig. 5. Right valve. Fig. 6. Left valve. Fig. 7. Line drawing: areolae arrangement. (Figs. 1-4,7 after Faust 1990b)
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FIGS. 11-16. Prorocentrum elegans sp. nov. FIG 11. Cells are oval in valve view; the cell surface is smooth with few valve pores. The two flagella are not present. FIG.12. Left valve margin at the anterior end is flat or inclined. FIG.13. Cells are ovate in side view. The periflagellar area is large relative to the cell size. It has a flagellar (F) and auxiliary (A) pore and an angled flagellar plate (arrowhead adjacent to the auxiliary pore). Large pores (arrow) and small pores (arrowheads) are present on the valves. The small pores are better illustrated at higher magnification in Figure 15. FIG.14. Periflagellar area is detached from the right valve. It has a smooth inner surface, and discrete platelets are unequal in size. FIG.15. The intercalary band is transversely striated, and the inner surface appears ribbed. Small pores (arrowheads) are situated in an array along the intercalary band. FIG.16. The two apical pores are separated by a ridge in this naked cell. Scale bars = 5 µm.
Note:Holotype SEM negative # 86075A; SEM stub #86; Field # n.a; Accession # 407164; Catalog # 44; Figure # 11.
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Figs. 1-4. Fig.1.Valve surface of Prorocentrum faustiae. Scale bar represents 5µm. Fig. 2. Intercalary band of Prorocentrum faustiae. Scale bar represents 5 um. Arrow heads point to the evenly spaced marginal pores. Fig. 3. Periflagellar area of Prorocentrum faustiae displays a large flagellar pore (F) and smaller auxiliary pore (A). Scale bar represents 1 µm. Arrow heads point to sutures which separate each periflagellar plate. Fig. 4. Periflagellar area in valve view. Scale bar represents 3 µm.
Note: Isolated from Heron Island, Australia (23.25° S, 151.55° E).
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Plate 41. Prorocentrum faustiae. Figs. 1-4. SEM. Fig. 1. Right valve. Cells broadly ovate to rotundate with slightly concave center. Valve surface rugose. Periflagellar area situated apically. Fig. 2. Left valve: apical region slightly excavated. Fig. 3. Intercalary band wide and transversely striated. Small marginal pores evenly spaced along cell perifery (arrows). Fig. 4. Periflagellar area: apical view. Broad V-shaped depression; larger flagellar pore (f) adjacent to smaller auxiliary pore (a). (All figures donated by S.L. Morton)
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Figs. 7-13. Prorocentrum foraminosum sp. nov. Fig.7. Right valve view showing the apical area, which has a narrow and shallow depression. The surface is covered with a larger number of pores but the centre is devoid of pores. Fig.8. The left valve has a flat anterior end. Left and right valves are similar in surface appearance. Fig.9. The surface is covered with scattered valve pores. Fig.10. The pores are round, equal in size and situated in shallow depressions with an opening in the centre. Fig.11. The intercalary band is smooth, cells have no marginal pores and cell shape is convex. Fig.12. The periflagellar area is narrowly triangular. The platelets appear vertical when viewed from the anterior end of the cell. There is one flagellar pore (F), one auxiliary pore (A) and 8 platelets of unequal size and shape. Fig. 13. Below the periflagellar area the flagellar opening (arrow) is observed in the cytoplasm.
EMu: HOLOTYPE SEM NEGATIVE # 23042; SEM STUB # 23; FIELD # 78-87; ACCESSION # 407159; CATALOG # 66: FIGURE # 7.