Longidorus carniolensis

Figure 1.Longidorus carniolensis sp. n. Female: A Neck region F Habitus H Pharyngeal bulb Male: G Habitus I Pharyngeal bulb; Juveniles: B–E Habitus of first, second, third and forth juvenile stages J–M Pharyngeal bulb of first, second, third and forth juvenile stages. Scale bars: B–G 1 mm; A, H–M 100 μm.

Figure 10.Longidorus carniolensis sp. n. Juvenile: A–D Anterior region of first, second, third and forth stages H–K Pharyngeal bulb of first, second, third and forth juvenile stages M, F, G, R genital primordium of first, second, third and forth stages N, S Tail shape of first stage O, T Tail shape of second stage P, U Tail shape of third stage Q, V Tail shape of forth stage Female: E Anterior region L Pharyngeal bulb W Tail shape. Scale bar: 50 μm.

Figure 11.Longidorus carniolensis sp. n. Scatter plot of the functional (, dark blue) and replacement odontostyle (, light blue) in relation to the body length of the juvenile stages and adults: females (, dark blue) and males (, red), female with very short odontostyle (, green).

Figure 12.Phylogenetic tree of rDNA D2/D3 expansion region sequences of Longidorus carniolensis sp. n. from Slovenia (square mark) and sequences of closely related Longidorus species (NCBI GenBank). Sequences were analysed using Neighbour Joining Method. Bootstrap support values higher than 50% are presented.

Figure 2.Longidorus carniolensis sp. n. Female: A–D Anterior ends E Vestigium in the walls of the slender part of pharynx F, G Vulval region G Anterior genital branch. Scale bars: A–I 50 μm, J 100 μm.

Figure 3.Longidorus carniolensis sp. n. Female: A Anterior region B–D Amphidial fovea E Vestigium F–H Vulval region I Vulval region, uterus and egg J Pharyngeal bulb, dorsal and subventral glands K, L Tail – different optical sections M Sphincter N Prerectum O–Q Variation in tail shape. Scale bars: I, N 200 μm; A–G, H–M, O–Q 50 μm.

Figure 4.Longidorus carniolensis sp. n. Male: A–E Anterior end B, D, E in sublateral view F Excretory pore and ventral pores G Partly protracted spicules H–I Tail end. Scale bar: 50 μm.

Figure 5.Longidorus carniolensis sp. n. Male: A, B Variation in tail shape. Scale bar: 50 μm.

Figure 6.Longidorus carniolensis sp. n. Male: A Anterior region B, C Head region D–G Amphidial fovea H Vestigium (white arrow), excretory pore (thick arrow) and ventral pores (slender arrows) I Ejaculatory glands (marked by arrows) J Lateral field K, L Pharyngeal bulb with glandular bodies (marked by arrows) M, N Sperm cells at different stage of development. Scale bars: A 200 μm; B–N 50 μm.

Figure 7.Longidorus carniolensis sp. n. Male: A Posterior genital branch B, C, E, F Tail and copulatory apparatus – different optical sections D, G Posterior end H Rectum (marked by arrow), spicules and lateral piece I Partly protracted spicules. Scale bars: A, D, G – 200 μm; B, C, E–F, H, I – 50 μm.

Figure 8.Longidorus carniolensis sp. n. Juveniles: A Neck region of first stage B–D Spear region of second, third and fourth stage. Scale bar: 50 μm.

Figure 9.Longidorus carniolensis sp. n. Evolution of the tail. A–D Tail of first–fourth juvenile stage E Tail of female. Scale bar: 100 μm.