Цевчести црви

Figure 1.Longidorus carniolensis sp. n. Female: A Neck region F Habitus H Pharyngeal bulb Male: G Habitus I Pharyngeal bulb; Juveniles: B–E Habitus of first, second, third and forth juvenile stages J–M Pharyngeal bulb of first, second, third and forth juvenile stages. Scale bars: B–G 1 mm; A, H–M 100 μm.

Figure 1.Crassolabium persicum sp. n. (all images are in lateral view) A Anterior region B Lip region and amphid fovea in surface C Pharyngeal expansion D Vagina E Spicules and lateral guiding piece F Female, posterior body region G Female, anterior genital branch H Male, posterior body region I Male, entire J Female, entire.

Figure 1.Longidorus cholevae sp. n. Female: A Anterior end F Habitus I Pharyngeal bulb J Anterior genital branch Male: G Habitus H Pharyngeal bulb Juveniles: B–E Habitus of first-, second-, third- and fourth-stage juveniles. Scale-bars: A, H, I, J 50 μm; B–G 1 mm.

Figure 2.Calcaridorylaimus castaneae sp. n. Female: A Pharyngeal gland nuclei B Anterior region C Pharyngeal region D Genital system E Vulval region F Sperm cells in uterus. Scale bars: A, B, E, F – 30 μm; C, D – 50 μm.

Figure 2.Longior longior Morffe & García sp. n. (female). A Esophageal region B Cephalic end C Tail, ventral view D Vulva E Egg F Genital tract G Entire nematode, lateral view.

Öesophagostomum sp. adult femalePosterior end of a female Oesophagostomum sp., showing the pointed tail.From CDC DPDx website

Figure 10.Longidorus carniolensis sp. n. Juvenile: A–D Anterior region of first, second, third and forth stages H–K Pharyngeal bulb of first, second, third and forth juvenile stages M, F, G, R genital primordium of first, second, third and forth stages N, S Tail shape of first stage O, T Tail shape of second stage P, U Tail shape of third stage Q, V Tail shape of forth stage Female: E Anterior region L Pharyngeal bulb W Tail shape. Scale bar: 50 μm.

Figure 2.Crassolabium persicum sp. n. (light micrographs all in lateral view).A Female, entire B Anterior region C Pharyngo–intestinal junction D Female, genital system E Neck region F Male, entire G Male, posterior region H Vagina I Female, caudal region J Spicules K Lateral guiding piece L Oviduct–uterus junction M Lateral chord and pores. (Scale bars: A, F – 500 µm; B, H, K – 10 µm; C, G – 50 µm; D, E – 100 µm; I, J, L, M – 20 µm).

Figure 9.Longidorus cholevae sp. n. Scatter plot of the functional (˜, juveniles and adults, females in orange) and replacement (™, juveniles) odontostyle in relation to body length of the juvenile developmental stages and adults.

Figure 3.Calcaridorylaimus castaneae sp. n. Female: A Posterior region. Male: B, C Extruded spicules with supplements D Posterior region. Scale bar: A–D – 30 μm.

Figure 3.Longior longior Morffe & Garcíasp. n. (male). A Entire nematode, lateral view B Esophageal region, lateral view C Cephalic end D Tail, lateral view.

Male Oesophagostomum sp. Posterior end of male Oesophagostomum sp. showing the bursa. Note the spicule (SP).From CDC DPDx Website

Figure 11.Longidorus carniolensis sp. n. Scatter plot of the functional (, dark blue) and replacement odontostyle (, light blue) in relation to the body length of the juvenile stages and adults: females (, dark blue) and males (, red), female with very short odontostyle (, green).

Figure 10.Phylogenetic relationships of Longidorus cholevae sp. n. and its closest species for the D2-D3 rDNA. Bayesian Inference strict consensus tree acquired under GTR+G model. Numbers at the nodes indicating posterior probabilities higher that 0.8 and bootstrap values more that 70% for ML and NJ are presented.

Figure 4.Calcaridorylaimus castaneae sp. n. Female: A Anterior end C Amphid F–I Tail shapes Male B Anterior end D Posterior end with extruded spicules, arrow indicating the spur E Posterior end. Scale bars: A, B, D–I – 20 μm; C – 6 μm.

Figure 5. Longior longior Morffe & García sp. n. (female). A Cephalic end B Egg. Longior longior Morffe & García sp. n. (male) C Cephalic end D Tail, lateral view E Pre-cloacal median mammiform papilla, lateral view F Post-cloacal dorso-lateral papilla. Longior similis Morffe, García & Ventosa, 2009 (male) G Cephalic end H Tail, lateral view. Scale bars: A, B, C, D, G, H. 0.05 mm. E, F. 0.020 mm.

Öesophagostomum adultMagnification of the anterior end. Note the presence of the cephalic vesicle (CV), cephalic groove (CG) and esophagus (ES).From CDC DPDx website

Figure 12.Phylogenetic tree of rDNA D2/D3 expansion region sequences of Longidorus carniolensis sp. n. from Slovenia (square mark) and sequences of closely related Longidorus species (NCBI GenBank). Sequences were analysed using Neighbour Joining Method. Bootstrap support values higher than 50% are presented.

Figure 11.Phylogenetic relationships of Longidorus cholevae sp. n. and its closest species for the partial 18S-ITS1 rDNA regions. Bayesian Inference strict consensus tree acquired under K2+G model. Numbers at the nodes indicating posterior probabilities higher that 0.8 and bootstrap values more that 70% for ML and NJ are presented.

Figure 5.Calcaridorylaimus castaneae sp. n. Female: A Entire body B Lip region D Prerectum, arrow pointing tongue-like valve E Pharyngeal bulb F Vulval region with posterior uterus G Vulval region with egg in posterior uterus H, I Vulval region J Cardia L Lateral field. Male: A Entire body C Lip region K Cardia M Supplements. Scale bars: A – 200 μm; B, C, M – 6 μm; E–L – 20 μm.

OesophagostomumL3 larva of Oesophagostomum sp., obtained via coproculture from the feces of a baboon (Papio ursinus) in South Africa. Note the long, thin, pointed tail. Image courtesy of the UTC Baboon Research Unit, University of Cape Town, South Africa.The definitive hosts of Oesophagostomum spp. become infected from the ingestion of infective L3 larvae, which develop in the environment. L3 larvae are longer than the L3 larvae of hookworms, measuring 710-950 µm in length. The intestine is made-up of alternating triangular-shaped cells (zigzag gut cells). The tail end has a long, thin, tapered sheath, with a considerable gap between the end of the tail and the end of the sheath.From CDC-DPDx

Figure 2.Longidorus carniolensis sp. n. Female: A–D Anterior ends E Vestigium in the walls of the slender part of pharynx F, G Vulval region G Anterior genital branch. Scale bars: A–I 50 μm, J 100 μm.

Figure 6.Calcaridorylaimus castaneae sp. n. Male: A Posterior end B Sperm cells in testis C–F Spicular region C Lateral piece of spicules D, E Extruded spicules, arrows pointing the spur F Spicules in the body. Scale bars: A, B – 20 μm; C–F – 18 μm.

ÖesophagostomumPost-Mortem Pathology Plate borrowed from Brumpti and Thomas. "The Pathological Report of Oesophagostomiasis in Man."Colon and small intestine tissue samples from patient infected with Oesophagostomum. Important to note are the nodules lining the lumen of the colon (right).From: "Introduction to the parasite family Oesophagostomum" (Stanford University Human Biology 103 class)