Otus asio

are eaten by

• Global Biotic Interactions: Pantherophis obsoletus (Say ex James 1823) (Western Ratsnake)
• Global Biotic Interactions: Bassariscus astutus (Lichtenstein 1830) (Ringtail)
• Global Biotic Interactions: Didelphis virginiana Kerr 1792 (Virginia Opossum)
• Global Biotic Interactions: Procyon lotor (Linnaeus 1758) (Northern Raccoon)

are host of

• Global Biotic Interactions: Procyrnea excisiformis
• Global Biotic Interactions: Procyrnea excisiformis
• Global Biotic Interactions: Procyrnea excisiformis
• Global Biotic Interactions: Hantavirus FL-2014
• Global Biotic Interactions: Hantavirus FL-2014

are parasitized by

• Global Biotic Interactions: Physocephalus sexalatus (Molin 1860)
• Global Biotic Interactions: Hamatospiculum cylindricum (Zeder 1803)
• Global Biotic Interactions: Cryptocotyle lingua (Creplin 1825)

eat

• Global Biotic Interactions: Diadophis punctatus (Linnaeus 1766) (Ring-necked Snake)

prey on

• Global Biotic Interactions: Myotis lucifugus (Le Conte 1831) (little brown bat)
• Global Biotic Interactions: Myotis grisescens A. H. Howell 1909 (Gray Myotis)