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  • Profile picture of Kari Pihlaviita who took this action.

    Kari Pihlaviita added the Finnish common name "Salmonellat" to "Salmonella SopEphi".

    8 months ago

  • Profile picture of Cyndy Parr who took this action.

    Cyndy Parr commented on "Salmonella SopEphi":

    @John McQuiston: Just learned of these comments you made. Would you like to add the information directly to the page content rather than keep them in comments? Just sign into EOL, go to the "Detail" tab and click "Add an article." Or, we can do it for you.

    over 1 year ago

  • Profile picture of Life on Earth who took this action.

    Life on Earth added text to "Brief Summary" on "Salmonella SopEphi".

    Salmonella infects around 142,000 people in the United States every year....

    about 2 years ago

  • Profile picture of John McQuiston who took this action.

    John McQuiston commented on "Salmonella SopEphi":

    The National Salmonella Reference Laboratory at the U.S. Centers for Disease Control and Prevention has determined that the new species of Salmonella subterranea reported by Shebolina et.al (4) is not likely a Salmonella species, demonstrated by molecular and phylogenetic evaluation. Methods including DNA-DNA hybridization, DNA sequencing followed by phylogenetic analysis of housekeeping genes and attempted PCR amplification of Salmonella specific genes all found this species, while likely new, is more closely related to the genus Escherichia. The WHO collaborating center for Salmonella also has evidence demonstrating that this new species is not likely Salmonella. http://www.pasteur.fr/sante/clre/cadrecnr/salmoms/WKLM_2007.pdf The initial report on this new species relied heavily on 16s rRNA sequencing which has been shown to be unreliable in the genus Enterobacteriaceae (2, 3). In that regard, the reported 16S similarity was within 0.1% for two different genera; their 16S sequence was 96.4% similar to S. bongori and 96.3% similar to Enterobacter cloacae. The phenotypic profile for the strain differed from Salmonella in three characteristics that are key for Salmonella identification, hydrogen sulfide production on TSI, indole production, and lysine utilization (1). The published phenotypic profile for the strain produced a best match with Escherichia hermanii using publically available probabilistic identification algorithms ( http://www.som.soton.ac.uk/staff/tnb/pib.htm) 1.Ewing, W. H. 1986. Edwards and Ewing’s identification of Enterobacteriaceae, 4th ed, vol. Burgess Publishing Co, New York., NY. 2.Janda, J. M., and S. L. Abbott. 2007. 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. J Clin Microbiol 45:2761-4. 3.Naum, M., E. W. Brown, and R. J. Mason-Gamer. 2008. Is 16S rDNA a reliable phylogenetic marker to characterize relationships below the family level in the enterobacteriaceae? J Mol Evol 66:630-42. 4.Shelobolina, E. S., S. A. Sullivan, K. R. O'Neill, K. P. Nevin, and D. R. Lovley. 2004. Isolation, characterization, and U(VI)-reducing potential of a facultatively anaerobic, acid-resistant Bacterium from Low-pH, nitrate- and U(VI)-contaminated subsurface sediment and description of Salmonella subterranea sp. nov. Appl Environ Microbiol 70:2959-65.

    almost 5 years ago

  • Profile picture of John McQuiston who took this action.

    John McQuiston commented on "Salmonella subterranea Shelobolina et al. 2005":

    The National Salmonella Reference Laboratory at the U.S. Centers for Disease Control and Prevention has determined that the new species of Salmonella subterranea reported by Shebolina et.al (4) is not likely a Salmonella species, demonstrated by molecular and phylogenetic evaluation. Methods including DNA-DNA hybridization, DNA sequencing followed by phylogenetic analysis of housekeeping genes and attempted PCR amplification of Salmonella specific genes all found this species, while likely new, is more closely related to the genus Escherichia. The WHO collaborating center for Salmonella also has evidence demonstrating that this new species is not likely Salmonella. http://www.pasteur.fr/sante/clre/cadrecnr/salmoms/WKLM_2007.pdf The initial report on this new species relied heavily on 16s rRNA sequencing which has been shown to be unreliable in the genus Enterobacteriaceae (2, 3). In that regard, the reported 16S similarity was within 0.1% for two different genera; their 16S sequence was 96.4% similar to S. bongori and 96.3% similar to Enterobacter cloacae. The phenotypic profile for the strain differed from Salmonella in three characteristics that are key for Salmonella identification, hydrogen sulfide production on TSI, indole production, and lysine utilization (1). The published phenotypic profile for the strain produced a best match with Escherichia hermanii using publically available probabilistic identification algorithms ( http://www.som.soton.ac.uk/staff/tnb/pib.htm) 1.Ewing, W. H. 1986. Edwards and Ewing’s identification of Enterobacteriaceae, 4th ed, vol. Burgess Publishing Co, New York., NY. 2.Janda, J. M., and S. L. Abbott. 2007. 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: pluses, perils, and pitfalls. J Clin Microbiol 45:2761-4. 3.Naum, M., E. W. Brown, and R. J. Mason-Gamer. 2008. Is 16S rDNA a reliable phylogenetic marker to characterize relationships below the family level in the enterobacteriaceae? J Mol Evol 66:630-42. 4.Shelobolina, E. S., S. A. Sullivan, K. R. O'Neill, K. P. Nevin, and D. R. Lovley. 2004. Isolation, characterization, and U(VI)-reducing potential of a facultatively anaerobic, acid-resistant Bacterium from Low-pH, nitrate- and U(VI)-contaminated subsurface sediment and description of Salmonella subterranea sp. nov. Appl Environ Microbiol 70:2959-65.

    almost 5 years ago

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