Pezizomycotina

Figure 1.A–F Jahnula purpurea (from the HOLOTYPE; MJF 14016, ILLS 72402). A–C Ascomata on submerged wood. Note the purple stain. Arrowheads indicate the subtending superficial hyphae on wood, which connect multiple ascomata on wood D Ascoma in water showing broad hyphae emerging from the base of the fruiting body E, F Longitudinal section through ascoma. Note broad pseudoparenchymatic cells comprising the peridial wall. Scale bars: A, C = 500 µm; B = 1 mm; D = 100 µm; E–F = 20 µm;

Figure 2.G–I Clavate to obclavate asci. J, K Ascus apex showing faint truncate ocular chamber L Pseudoparaphyses M–N Multiguttulate brown ascospores. Note ascospores showing minutely verrucose warts forming a loose reticulate pattern O Immature ascospore in India ink. Scale bars: G–I, M = 20 µm; J–L = 5 µm; N, O = 10 µm.

Figure 1.Roesleria subterranea (TNS-F-38701). 1 Growing habits of two mature mazaedia on the root tip of an unidentified tree 2 Hymenium of mazaedium, showing protruding paraphyses 3 Enlarged hymenium showing developmental stages of asci and paraphyses 4 Mature ascus containing eight ascospores 5 Paraphysis 6 Discharged matured ascospores; on the right, a transversely one-septate spore is shown in side view 7 Germination of ascospores on MEYE, showing that germination tubes from one of the two-celled spores 2–6 Mounted in Meltzer’s solution 7 Mounted in lacto-aniline blue. Bars: 1 = 2 mm; 2 = 50 ?m; 3–7 = 10 ?m.

Figure 2.A single most parsimonious tree of Roesleria subterranea (ITS-5.8S sequences). Tree length = 31, CI = 0.8065, HI = 0.1935, RI = 0.8723, RC = 0.7035. The tree was constructed from a dataset of all available ITS-5.8S sequences of Roesleria subterranea from various hosts and localities. The numbers on the branches indicate the bootstrap values (BP) of 1000 replications in maximum parsimony analysis when the BP exceeds 50%. The scale bar indicates the number of substitutions. Abbreviations indicate the localities of the isolates: AU, Austria; GE, German; IT, Italy; NE, Netherlands; US, USA; JP, Japan.